The C2 protein of tomato yellow leaf curl Sardinia virus acts as a pathogenicity determinant and a 16-amino acid domain is responsible for inducing a hypersensitive response in plants.
Identifieur interne : 000269 ( Main/Exploration ); précédent : 000268; suivant : 000270The C2 protein of tomato yellow leaf curl Sardinia virus acts as a pathogenicity determinant and a 16-amino acid domain is responsible for inducing a hypersensitive response in plants.
Auteurs : Slavica Mati [Italie] ; Mattia Pegoraro [Italie] ; Emanuela Noris [Italie]Source :
- Virus research [ 1872-7492 ] ; 2016.
Descripteurs français
- KwdFr :
- Agrobacterium (génétique), Arabidopsis (immunologie), Arabidopsis (virologie), Begomovirus (pathogénicité), Cellules végétales (physiologie), Cellules végétales (virologie), Expression des gènes (MeSH), Facteurs de virulence (génétique), Facteurs de virulence (métabolisme), Interactions hôte-pathogène (MeSH), Maladies des plantes (immunologie), Maladies des plantes (virologie), Mort cellulaire (MeSH), Protéines virales (génétique), Protéines virales (métabolisme), Tabac (immunologie), Tabac (virologie), Transformation génétique (MeSH).
- MESH :
- génétique : Agrobacterium, Facteurs de virulence, Protéines virales.
- immunologie : Arabidopsis, Maladies des plantes, Tabac.
- métabolisme : Facteurs de virulence, Protéines virales.
- pathogénicité : Begomovirus.
- physiologie : Cellules végétales.
- virologie : Arabidopsis, Cellules végétales, Maladies des plantes, Tabac.
- Expression des gènes, Interactions hôte-pathogène, Mort cellulaire, Transformation génétique.
English descriptors
- KwdEn :
- Agrobacterium (genetics), Arabidopsis (immunology), Arabidopsis (virology), Begomovirus (pathogenicity), Cell Death (MeSH), Gene Expression (MeSH), Host-Pathogen Interactions (MeSH), Plant Cells (physiology), Plant Cells (virology), Plant Diseases (immunology), Plant Diseases (virology), Tobacco (immunology), Tobacco (virology), Transformation, Genetic (MeSH), Viral Proteins (genetics), Viral Proteins (metabolism), Virulence Factors (genetics), Virulence Factors (metabolism).
- MESH :
- chemical , genetics : Viral Proteins, Virulence Factors.
- genetics : Agrobacterium.
- immunology : Arabidopsis, Plant Diseases, Tobacco.
- chemical , metabolism : Viral Proteins, Virulence Factors.
- pathogenicity : Begomovirus.
- physiology : Plant Cells.
- virology : Arabidopsis, Plant Cells, Plant Diseases, Tobacco.
- Cell Death, Gene Expression, Host-Pathogen Interactions, Transformation, Genetic.
Abstract
The role of the C2 protein in the pathogenicity of tomato yellow leaf curl Sardinia virus (TYLCSV) was investigated. Here we report that Agrobacterium-mediated transient expression of TYLCSV C2 resulted in a strong hypersensitive response (HR) in Nicotiana benthamiana, N. tabacum, and Arabidopsis thaliana, with induction of plant cell death and production of H2O2. Since HR is not evident in plants infected by TYLCSV, it is expected that TYLCSV encodes a gene (or genes) that counters this response. HR was partially counteracted by co-agroinfiltration of TYLCSV V2 and Rep, leading to chlorotic reaction, with no HR development. Considering that the corresponding C2 protein of the closely related tomato yellow leaf curl virus (TYLCV) did not induce HR, alignment of the C2 proteins of TYLCSV and TYLCV were carried out and a hypervariable region of 16 amino acids was identified. Its role in the induction of HR was demonstrated using TYLCSV-TYLCV C2 chimeric genes, encoding two TYLCSV C2 variants with a complete (16 aa) or a partial (10 aa only) swap of the corresponding sequence of TYLCV C2. Furthermore, using NahG transgenic N. benthamiana lines compromised in the accumulation of salicylic acid (SA), a key regulator of HR, only a chlorotic response occurred in TYLCSV C2-infiltrated tissue, indicating that SA participates in such plant defense process. These findings demonstrate that TYLCSV C2 acts as a pathogenicity determinant and induces host defense responses controlled by the SA pathway.
DOI: 10.1016/j.virusres.2016.01.014
PubMed: 26826600
Affiliations:
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Here we report that Agrobacterium-mediated transient expression of TYLCSV C2 resulted in a strong hypersensitive response (HR) in Nicotiana benthamiana, N. tabacum, and Arabidopsis thaliana, with induction of plant cell death and production of H2O2. Since HR is not evident in plants infected by TYLCSV, it is expected that TYLCSV encodes a gene (or genes) that counters this response. HR was partially counteracted by co-agroinfiltration of TYLCSV V2 and Rep, leading to chlorotic reaction, with no HR development. Considering that the corresponding C2 protein of the closely related tomato yellow leaf curl virus (TYLCV) did not induce HR, alignment of the C2 proteins of TYLCSV and TYLCV were carried out and a hypervariable region of 16 amino acids was identified. Its role in the induction of HR was demonstrated using TYLCSV-TYLCV C2 chimeric genes, encoding two TYLCSV C2 variants with a complete (16 aa) or a partial (10 aa only) swap of the corresponding sequence of TYLCV C2. Furthermore, using NahG transgenic N. benthamiana lines compromised in the accumulation of salicylic acid (SA), a key regulator of HR, only a chlorotic response occurred in TYLCSV C2-infiltrated tissue, indicating that SA participates in such plant defense process. These findings demonstrate that TYLCSV C2 acts as a pathogenicity determinant and induces host defense responses controlled by the SA pathway. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26826600</PMID><DateCompleted><Year>2016</Year><Month>12</Month><Day>13</Day></DateCompleted><DateRevised><Year>2016</Year><Month>12</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1872-7492</ISSN><JournalIssue CitedMedium="Internet"><Volume>215</Volume><PubDate><Year>2016</Year><Month>Apr</Month><Day>02</Day></PubDate></JournalIssue><Title>Virus research</Title><ISOAbbreviation>Virus Res</ISOAbbreviation></Journal><ArticleTitle>The C2 protein of tomato yellow leaf curl Sardinia virus acts as a pathogenicity determinant and a 16-amino acid domain is responsible for inducing a hypersensitive response in plants.</ArticleTitle><Pagination><MedlinePgn>12-9</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.virusres.2016.01.014</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0168-1702(16)30054-5</ELocationID><Abstract><AbstractText>The role of the C2 protein in the pathogenicity of tomato yellow leaf curl Sardinia virus (TYLCSV) was investigated. Here we report that Agrobacterium-mediated transient expression of TYLCSV C2 resulted in a strong hypersensitive response (HR) in Nicotiana benthamiana, N. tabacum, and Arabidopsis thaliana, with induction of plant cell death and production of H2O2. Since HR is not evident in plants infected by TYLCSV, it is expected that TYLCSV encodes a gene (or genes) that counters this response. HR was partially counteracted by co-agroinfiltration of TYLCSV V2 and Rep, leading to chlorotic reaction, with no HR development. Considering that the corresponding C2 protein of the closely related tomato yellow leaf curl virus (TYLCV) did not induce HR, alignment of the C2 proteins of TYLCSV and TYLCV were carried out and a hypervariable region of 16 amino acids was identified. Its role in the induction of HR was demonstrated using TYLCSV-TYLCV C2 chimeric genes, encoding two TYLCSV C2 variants with a complete (16 aa) or a partial (10 aa only) swap of the corresponding sequence of TYLCV C2. Furthermore, using NahG transgenic N. benthamiana lines compromised in the accumulation of salicylic acid (SA), a key regulator of HR, only a chlorotic response occurred in TYLCSV C2-infiltrated tissue, indicating that SA participates in such plant defense process. These findings demonstrate that TYLCSV C2 acts as a pathogenicity determinant and induces host defense responses controlled by the SA pathway. </AbstractText><CopyrightInformation>Copyright © 2016 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Matić</LastName><ForeName>Slavica</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Institute for Sustainable Plant Protection, CNR, Strada delle Cacce 73, 10135 Torino, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pegoraro</LastName><ForeName>Mattia</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Institute for Sustainable Plant Protection, CNR, Strada delle Cacce 73, 10135 Torino, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Noris</LastName><ForeName>Emanuela</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Institute for Sustainable Plant Protection, CNR, Strada delle Cacce 73, 10135 Torino, Italy. Electronic address: emanuela.noris@ipsp.cnr.it.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>01</Month><Day>28</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Virus Res</MedlineTA><NlmUniqueID>8410979</NlmUniqueID><ISSNLinking>0168-1702</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014764">Viral Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D037521">Virulence Factors</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D060054" 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MajorTopicYN="N">physiology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014764" MajorTopicYN="N">Viral Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D037521" MajorTopicYN="N">Virulence Factors</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Agroinfiltration</Keyword><Keyword MajorTopicYN="N">C2</Keyword><Keyword MajorTopicYN="N">Hypersensitive response</Keyword><Keyword MajorTopicYN="N">Pathogenicity determinant</Keyword><Keyword MajorTopicYN="N">Salicylic acid</Keyword><Keyword MajorTopicYN="N">TYLCSV</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>10</Month><Day>31</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2016</Year><Month>01</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>01</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>1</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>1</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>12</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26826600</ArticleId><ArticleId IdType="pii">S0168-1702(16)30054-5</ArticleId><ArticleId IdType="doi">10.1016/j.virusres.2016.01.014</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Italie</li></country><region><li>Piémont</li></region><settlement><li>Turin</li></settlement></list><tree><country name="Italie"><region name="Piémont"><name sortKey="Mati, Slavica" sort="Mati, Slavica" uniqKey="Mati S" first="Slavica" last="Mati">Slavica Mati</name></region><name sortKey="Noris, Emanuela" sort="Noris, Emanuela" uniqKey="Noris E" first="Emanuela" last="Noris">Emanuela Noris</name><name sortKey="Pegoraro, Mattia" sort="Pegoraro, Mattia" uniqKey="Pegoraro M" first="Mattia" last="Pegoraro">Mattia Pegoraro</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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